Objective: To study eight types of sexual assault among cisgender women, cisgender men and gender minority college students, estimating the prevalence of perpetration by persons of particular gender identities. Participants and Methods: 13,685 sexual assault survivors were surveyed. For each type of sexual assault victimization experienced by cisgender women, cisgender men and gender minorities, prevalence estimates assessed the extent of perpetration by persons of each gender identity. Results: Cisgender men were the most prevalent perpetrators of all types of sexual assault against cisgender women and gender minorities. Cisgender women were the most prevalent perpetrators of five types of sexual assault against cisgender men, with cisgender women and cisgender men being similarly likely to perpetrate three types of sexual assault against cisgender men. Conclusions: Cisgender men perpetrate most sexual assaults against cisgender women and gender minority students; cisgender women and cisgender men perpetrate most sexual assaults against cisgender men students.