Wheat powdery mildew is an important fungal pathogen of wheat with an obligatory biotrophic lifestyle (a parasite that can only develop on a living host). We investigated the genetics of this host-pathogen interaction by using phenotyping and PCR assays to detect genes in both wheat and powdery mildew, which are known determinants of the outcome…

Course-based undergraduate research experiences (CUREs) provide students with valuable opportunities to engage in research in a classroom setting, expanding access to research opportunities for undergraduates, fostering inclusive research and learning environments, and bridging the gap between the research and education communities. While…

Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from…

Plant awareness disparity (PAD, formerly plant blindness) is the tendency not to notice plants in one's environment, which leads to the perspective that plants are unimportant. In this paper, we explore how plants and animals are represented in introductory biology textbooks to determine if these texts are contributing to PAD in undergraduate…

We used a novel approach that combined direct PCR of plant tissue and SRAP DNA fingerprinting to seek to identify the crosses made in "Brassica rapa" used in the Wisconsin Fast Plants. Our goal was to develop a rapid method for students to use in a biology, botany, or genetics class to identify the parental types (purple stem, non-purple…

The current and ongoing challenges brought on by climate change will require future scientists who have hands-on experience using advanced molecular techniques, can work with large data sets, and can make correlations between metadata and microbial diversity. A course-embedded research project can prepare students to answer complex research…

The ability of living organisms to respond and adapt to a changing climate is an urgent concern. However, current educational efforts aimed at increasing US undergraduate student climate literacy primarily focus on the causes of, and abiotic responses to, climate change, mostly neglecting the biotic impacts. Here, we present a new framework, the…

Soft rot diseases cause crop losses all over the world. These losses can have large economic impacts and threaten international food security. Soft rot bacterial pathogens produce pectolytic enzymes that attack the pectin around plant cells resulting in smelly, wet, and mushy tissue maceration. Once symptoms are visible, the crop cannot be used or…

Humans have long sought to understand the forces that shape the diversity of living creatures on our planet. This work is primarily concerned with examining one such force--the historical action of natural selection--from practical, philosophical, and pedagogical perspectives. The first half is concerned with a suite of genetic mechanisms in…

DNA barcoding is an important molecular methodology for species identification that was developed over the last two decades and it should be covered in the biology bachelor curriculum. Here, we present an example of DNA barcoding by sequencing a segment of the 28S nuclear ribosomal large subunit rRNA gene of wild mushrooms and framing the…

The coffee bean is the most important agricultural product in international trade; it ranks second behind petroleum in product exports worldwide. An estimated 15 billion coffee trees are being grown to meet this demand, and the work that goes into this directly contributes to the income of 25 million small producers worldwide. Due to the…

A critical thinking assessment was used to compare biology students in a nonmajors course who participated in either a multisemester research program or in a one-semester lab study. Initially, it was thought the former would be more effective in promoting critical thinking improvement than the latter. Data from nine biology classes with pre- and…

A classroom exercise is described in which college students take part in creating and supporting an evolutionary hypothesis that explains effort grunting. The exercise holds their interest throughout and readies them to understand hypotheses of animal and plant evolution. It informs them about the dependence of cultural evolution upon biological…

Teaching biological evolution can be difficult on a number of levels, be it student confusion arising from prior conceptions and the controversy surrounding evolution, or simply because the material is complex. Games and simulations can help to convey complex topics and also to increase variety in teaching methods. Here I describe a card game that…

Next-generation sequencing (NGS)-based methods are revolutionizing biology. Their prevalence requires biologists to be increasingly knowledgeable about computational methods to manage the enormous scale of data. As such, early introduction to NGS analysis and conceptual connection to wet-lab experiments is crucial for training young scientists.…