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Problem-Solving Test: Analysis of DNA Damage Recognizing Proteins in Yeast and Human Cells
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2013
The experiment described in this test was aimed at identifying DNA repair proteins in human and yeast cells. Terms to be familiar with before you start to solve the test: DNA repair, germline mutation, somatic mutation, inherited disease, cancer, restriction endonuclease, radioactive labeling, [alpha-[superscript 32]P]ATP, [gamma-[superscript…
Descriptors: Genetics, Science Experiments, Multiple Choice Tests, Problem Solving
Problem-Solving Test: The Mechanism of Transcription Termination by the Rho Factor
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2012
Transcription termination comes in two forms in "E. coli" cells. Rho-dependent termination requires the binding of a termination protein called Rho factor to the transcriptional machinery at the terminator region, whereas Rho-independent termination is achieved by conformational changes in the transcript itself. This article presents a test…
Descriptors: Biology, Science Instruction, Science Experiments, Molecular Biology
Analysis of Chromatin Organisation
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2011
Terms to be familiar with before you start to solve the test: chromatin, nucleases, sucrose density gradient centrifugation, melting point, gel electrophoresis, ethidium bromide, autoradiography, Southern blotting, Northern blotting, Sanger sequencing, restriction endonucleases, exonucleases, linker DNA, chloroform extraction, nucleosomes,…
Descriptors: Genetics, Science Instruction, Scientific Concepts, Animals
Problem-Based Test: An "In Vitro" Experiment to Analyze the Genetic Code
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2010
Terms to be familiar with before you start to solve the test: genetic code, translation, synthetic polynucleotide, leucine, serine, filter precipitation, radioactivity measurement, template, mRNA, tRNA, rRNA, aminoacyl-tRNA synthesis, ribosomes, degeneration of the code, wobble, initiation, and elongation of protein synthesis, initiation codon.…
Descriptors: Genetics, Problem Solving, Molecular Biology, Laboratory Experiments
Problem-Solving Test: Restriction Endonuclease Mapping
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2011
The term "restriction endonuclease mapping" covers a number of related techniques used to identify specific restriction enzyme recognition sites on small DNA molecules. A method for restriction endonuclease mapping of a 1,000-basepair (bp)-long DNA molecule is described in the fictitious experiment of this test. The most important fact needed to…
Descriptors: Genetics, Problem Solving, Biochemistry, Molecular Biology
Problem-Solving Test: "In Vitro" Protein Kinase A Reaction
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Szeberenyi, Jozsef – Biochemistry and Molecular Biology Education, 2009
Phosphorylation of proteins by protein kinases is an important mechanism in the regulation of protein activity. Among hundreds of protein kinases present in human cells, PKA, the first kinase discovered, belongs to the most important and best characterized group of these enzymes. The author presents an experiment that analyzes the "in vitro"…
Descriptors: Molecular Biology, Biochemistry, Laboratory Experiments, Test Items