The purpose of this research is to investigate the relationship between sports and self-regulation and school burnout in high school students who are athletes and nonathletes. Relational screening method was used in the research. 387 high school students (188 athletes, 199 non-athletes) participated in the study. The average age of the participants was ± 15.9 years. The participating students were determined by the convenience sampling method. The data of the research were collected with the Personal Information Form, the Perceived Self-Regulation Scale and the School Burnout Scale. The data were analyzed by using the SPSS program, Independent Sample T-Test, One Way Anova Test, Tukey and Pearson Correlation tests. The significance level was accepted as p<0.05 in the analyses. According to the results of this research, the school burnout levels of non-athlete students are higher than those of athlete students. There is no significant relationship between athletes and self-regulation. The school burnout levels of athlete students who workout for 1-4 months and 9-12 months during the year are higher than those who workout for 5-8 months. On the other hand, female students' self-regulation levels are higher than males. The self-regulation and school burnout levels of the participants did not show a significant difference depending on the age.