The purpose of this study is to prepare a multiple-choice achievement test with high reliability and validity for the "Let's Solve the Puzzle of Our Body" unit. For this purpose, a multiple choice achievement test consisting of 46 items was applied to 178 fifth grade students in total. As a result of the test and material analysis performed during the test development process, difficulty, distinctiveness, and item-total correlation coefficients of the materials were calculated. For the validity study, a table of specifications was prepared and the Content Validity Index (CVI) was found to be 0.95 by taking an expert opinion. As a result of the analysis, 8 items were removed from the test and the KR-20 reliability coefficient of the final test consisting of 38 items was calculated as 0.87. As a result of the item analyses, while item difficulty indices were valued between 0.30 and 0.74, item distinctiveness indeces were valued between 0.31 and 0.71. The average difficulty of the test was calculated as moderate (0.56) and its distinctiveness was calculated as very good (0.49).