Career and Technical Education (CTE) Emerging Health Professional (EHP) programs are primarily populated by female students in the U.S. The development and testing of innovative educational models such as serious simulation games have been suggested for greater internalization of hand hygiene best practices behaviors for EHP students, but 50% of these female students do not play, or rarely play, games of any kind. This paper describes a hand hygiene serious simulation game which was intentionally designed for non-game-oriented grade 12 female CTE EHP students based upon the findings of two learner analyses. The deliberately informed design is expected to motivate these students to independently choose to persistently play to mastery level achievement by fostering their need for competence, autonomy, and relatedness, which should lead to greater internalization of hand hygiene compliant behaviors earlier in their preprofessional healthcare careers.