With the study reported on here, we aimed to determine the career barriers on organisational alienation and professional burnout levels of pre-school teachers and to reveal the relationship between their organisational alienation and burnout levels. A relational survey model was used in the study. The population of the study consisted of 896 pre-school teachers who worked in public and private schools in Mersin, Türkiye during the 2019-2020 academic year. The sample consisted of 309 pre-school teachers selected from the population using the convenience sampling method. The data were collected using the women's career barriers scale (Inandi, 2009a), Maslach burnout inventory (Ergin, 1992) and pre-school teachers professional alienation scale (Kihri, 2013). Correlation and regression analyses were performed on the data. As a result of the study, apart from social gender stereotypes and meaninglessness dimensions of alienation, positive and highly significant relationships were found between all sub-dimensions of career barriers and all sub-dimensions of alienation. Moreover, apart from social gender stereotypes and personal accomplishment sub-dimension of burnout, a positive and highly significant relationship between all sub-dimensions of career barriers and all sub-dimensions of burnout was found.