This study investigated the effects of two contrasting learning environments on students' course experiences: a lecture-based setting to a student-activating teaching environment. In addition, the evaluative treatment involved five research conditions that went together with one of four assessment modes, namely, portfolio, case-based, peer assessment, and multiple-choice testing. Data (N = 608) were collected using the Course Experience Questionnaire. Results showed that the instructional intervention (i.e. lectures versus student-activating treatment) influenced students' course experiences, but in the opposite direction to that expected. In declining order, the following scales (5 out of 7) revealed statistically significant differences: Clear Goals and Standards; the General scale; Appropriate Workload; Good Teaching; and Independence. Moreover, when the assessment mode was considered, also the Appropriate Assessment scale demonstrated significant differences between the five research conditions. Moreover, the same teaching/learning environments led to diverse students' perceptions. While the perceptions of lecture-taught students were focused and concordantly positive, students' course experiences with student-activating methods were widely varied and both extremely positive and negative opinions were present. Students' arguments in favor of the activating setting were the variety of teaching methods, the challenging and active nature of the assignments and the joys of collaborative work in teams, whereas students expressed dissatisfaction with the perceived lack of learning gains, the associated time pressure and workloads, and the (exclusive) use of collaborative assignments and related group difficulties.