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Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L. – Journal of Chemical Education, 2008
This laboratory project is one component of a semester-long advanced biochemistry laboratory course that uses several complementary techniques to study tRNA[superscript Phe] conformational changes induced by ligand binding. In this article we describe a set of experiments in which the thermal unfolding of tRNA[superscript Phe] is studied with…
Descriptors: Biochemistry, Laboratory Experiments, Science Laboratories, Science Experiments