The psychometric properties of a new, brief measure, the Revised Children's Manifest Anxiety Scale-Second Edition (RCMAS-2) Short Form, were examined in a sample of 1,003 U.S. elementary and secondary students. The RCMAS-2 Short Form consists of the first 10 items of the RCMAS-2. The results of confirmatory factor analysis indicated that the modified one-factor model provided an adequate fit for students in Grades 2 to 12. The results of tests of measurement invariance supported configural, weak, and strong invariance of the RCMAS-2 Short Form across gender and configural, weak, and partial strong invariance of the RCMAS-2 Short Form across age. Gender and age differences were also examined on the RCMAS-2 Short Form. The results of latent mean analyses indicated that adolescents (ages 12-19) and females had significantly higher factor means than children (ages 7-11) and males, respectively, on the RCMAS-2 Short Form. Moderate correlations between the RCMAS-2 Short Form scores and scores of measures of test anxiety were found. Implications of the findings for researchers and clinicians who work with children and adolescents are discussed.