Public speaking, especially in a foreign language, is associated with increased anxiety. Research has shown the potential of virtual reality (VR) for simulating real-life experiences, allowing for public speaking practice in an ecological and safe environment. This between-subjects study investigated the effect of VR on foreign language anxiety (FLA) in public speaking practice. Intermediate learners of English participated in eight public speaking sessions over a three-month period, yielding 160 research observations. The experimental intervention took place in high-immersion VR with subjects wearing a VR headset and speaking in front of virtual audience. In the control intervention, subjects used a videoconferencing platform (Zoom) to speak in front of a real-life audience. Multivariate regression analysis revealed that practicing speaking in VR was associated with statistically significant lower FLA scores, compared with speaking practice using Zoom. The study found that VR technology had a positive effect on practicing public speaking in a foreign language. The research findings have practical implications for professionals and curriculum designers in various domains where public speaking skills are essential. For example, incorporating VR-based public speaking practice can benefit professionals preparing for a job interview, an elevator pitch, or a conference presentation. Curriculum designers can consider integrating VR simulations into language courses to provide students with realistic public speaking experiences. This approach can help students overcome language barriers, reduce anxiety, and develop their communication skills in a controlled and supportive environment.