This publication was prepared for elementary teachers and other local personnel responsible for providing, maintaining and using living organisms to enhance elementary science programs. The manual contains a foreword, general information, and an appendix. It gives information concerning equipment and supplies, establishing and maintaining an aquarium, and culturing techniques. Techniques are given for culturing the following organisms: guppies and aquatic plants, land snails, chlamydomonas, daphnia, isopods, crickets, mealworm beetles, fruit flies, and wingless pea aphids. The techniques have been researched and developed in the Life Science Center of the Department of Science Education at East Carolina University and have been tried by preservice elementary teachers taking courses in science education. (LS)