Matrix training is a systematic teaching approach that can facilitate generalized language. Specific responses are taught that result in the emergence of untrained responses. This type of training facilitates the use of generalized language in children with autism spectrum disorder (ASD). This study used a matrix training procedure with a toddler with ASD. The participant was taught five responses that consisted of action-object instructions. He generalized responding to 11 untrained of 16 learned responses. This case study provided preliminary support for the use of a matrix training procedure with a toddler with ASD to promote generative language acquisition.