The aims of this research were to validate the Cultural Learning Environment Questionnaire in the Brunei context and to evaluate culturally sensitive factors (gender equity, collaboration, deference, competition, teacher authority, modelling and congruence) of upper secondary students' learning environment. The subjects of this study were 831 upper secondary science students from coeducational schools in Brunei. The data were collected by administering the Cultural Learning Environment Instrument developed by Fisher and Waldrip. Factor analysis and reliability results showed that the instrument was suitable for evaluating the six culturally sensitive factors associated with the cultural learning environment of Bruneian upper secondary students. The results also suggested that students believed both genders were treated equally in their classes. The students viewed that they were to some extent dependent learners; however, they were willing to give their independent views in their classes. The data also suggest that students perceived that they were equally cooperative and competitive. The culturally sensitive factors of the students' cultural learning environment were not influenced by their gender, but regional variations in values for some of these factors were observed. Further research has been recommended to investigate how the students were equally cooperative and competitive, as well as what factors contributed to the regional differences.