Oxygen consumption at peak physical exertion (VO[subscript 2] maximum) is the most widely used indicator of cardiorespiratory fitness. The purpose of this study was to compare two protocols for its estimation, cycle ergometer testing and the 20 m shuttle run, among children with and without probable developmental coordination disorder (pDCD). The shuttle run test was conducted during regular school hours, usually in the gymnasium. Children were then invited to a lab to complete the cycle ergometer protocol. Children were categorized as possible cases of DCD using the Movement-ABC-2. The analysis was performed using cut-points at both the 5th (n = 38) and 15th (n = 51) percentiles. The average age of children in the study was 12 years (SD = 0.5). Children with pDCD had poorer VO[subscript 2] maximum when compared to typically developing children based on both the shuttle run and the cycle ergometer. The correlation between tests is in the moderate to high range (r = 0.71, p less than 0.001); 0.78 for girls, and 0.73 for boys. The overall difference in correlations between typically developing children and children with pDCD based on the 15th percentile was 0.12 (p = 0.27). For children with pDCD based on the 5th percentile however, the difference between groups was larger (difference in r = 0.25), and was statistically significant (p = 0.02). In multivariate analyses, there was no difference in the effect of the shuttle run results in predicting VO[subscript 2] maximum obtained through the cycle ergometer test for children with pDCD compared to those without the condition. Regardless of the test, the patterns of association between children with pDCD and typically developing children were the same reinforcing the findings of previous field-based reports. Moderate to good correlations, at the 15th percentile cut-point, between tests suggests that the shuttle run test is a reliable substitute in this population when lab based assessments of VO[subscript 2] maximum are not feasible. (Contains 1 figure and 4 tables.