This study explored preservice teachers' views of their own cultural values, the cultural values they believed scientists hold, and the relationships of these views to their conceptions of nature of science (NOS). Parallel assignments in a foundations of early childhood education and a science methods course required preservice teachers to explore their own cultural backgrounds and their perceptions of the cultural backgrounds of scientists. The "Schwartz Values Inventory" was used to measure preservice teachers' personal cultural values and those they perceived of scientists. The "Views of Nature of Science version B" questionnaire and interviews assessed teachers' conceptions of NOS. Copies of student work were collected and sought for themes indicating how preservice teachers perceived scientists' cultural values and how those perceptions changed over time. We found that from the beginning to the end of the semester, preservice teachers perceived fewer differences between their own cultural values and those they perceived scientists held, though they did not change their own cultural values. We found that preservice teachers' NOS conceptions improved, and that they were related to both their cultural values and those they perceived scientists held. Preservice teachers who indicated the fewest differences between their own cultural values and those they perceived scientists held the most informed conceptions of NOS.