Enzymes are nature's catalysts, mediating chemical processes in living systems. The study of enzyme function and mechanism includes defining the maximum catalytic rate and affinity for substrate/s (among other factors), referred to as enzyme kinetics. Enzyme kinetics is a staple of biochemistry curricula and other disciplines, from molecular and…

Reported herein is a multidisciplinary laboratory experiment for advanced undergraduate students that includes elements of material synthesis, in the synthesis of cyclodextrin-containing metal-organic frameworks (CD-MOFs), and environmental chemistry, in the use of these MOFs for pollutant removal. This multiday laboratory experiment starts with…

In this organic chemistry laboratory experiment, students are guided through a series of exercises to understand the behavior of compounds during liquid-liquid extraction. Aspects of density, structure, solubility, acid-base theory, and pK[subscript a] are all incorporated into activities that students complete in groups. After using their initial…

Scanning probe-based microscopes (SPMs) are widely used in biology, chemistry, materials science, and physics to image and manipulate matter on the nanoscale. Unfortunately, high school and university departments lack expensive SPM tools and materials microscopy activities to educate a large number of students in this vital SPM imaging technique.…

The Internet of Things (IoT) is a network of objects that can exchange data with other devices also connected to the Internet. One of the most common consumer examples of IoT is home automation, as a variety of smart devices, including doorbells, lightbulbs, thermostats, and refrigerators are now available which users can control remotely using…

Considering the large number of magnetic stirring hot plates that are damaged or unused in chemical laboratories, this Technology Report presents guidelines for students to be able to diagnose problems, and repair and maintain magnetic stirring hot plates using common and low-cost parts, or even repurposed materials. In addition, this paper…

In astronomy, methods such as direct imaging or interferometry-based techniques (Michelson stellar interferometry for example) are used for observations. A particular advantage of interferometry is that it permits greater spatial resolution compared to direct imaging with a single telescope, which is limited by diffraction owing to the aperture of…

A simple bioassay was designed for the detection of adenosine, a human metabolite involved in several physiological processes, on the basis of the functionalization of gold nanoparticles with DNA oligonucleotides selectively binding adenosine. The test was possible thanks to the selective and sensitive binding of adenosine by two DNA…

An introductory guide to deducing the mechanism of chemical reactions is presented. Following a typical workflow for probing reaction mechanism, the guide introduces a wide range of kinetic and mechanistic tools. In addition to serving as a broad introduction to mechanistic analysis for students and researchers, the guide has also been used by…