Perspectives, policy issues, and options for Congressional action that relate most directly to the development and implementation of alternatives to animal use in research and testing are addressed in this report. Testimonies and reports include those from the Office of Technology Assessment, the National Institute of Health, and the Food and Drug Administration. Discussion focused on the prospects for replacing, reducing, and refining animal use. Alternatives identified include: (1) continued but modified use of animals; (2) use of living systems particularly the in-vitro culture of cells, tissues and organs; (3) use of nonliving systems that mimic biological functions; and (4) computer programs. (ML)