Studies on teaching methodologies have been one of the most analyzed research topics in Physical Education (PE). Many instruments have been created to examine the teaching methodologies in PE. This systematic review aimed to identify the tools used in peer-reviewed journals (until March 2022) that assessed teaching methodologies in PE. Articles selected for analysis were found in Web of Science, SCOPUS, PsycINFO, SportDiscus, and ERIC databases. Potential studies were located using different combinations of keywords related to teaching methodologies. Quality was checked through five criteria on a quality assessment list. A total of 63 instruments were extracted from 58 articles that evaluated teaching methodologies. This systematic review outlines the currently available instruments and their characteristics (i.e. author(s), name, variables/dimensions, items/categories, response format, sample, and type of instrument) to assess teaching methodologies in PE. Future works could use this review to employ and/or improve the existing tools.