Work and family are two of the most important parts in one's life. Individuals cannot fulfill their work requirements and family responsibilities at the same time, so they are faced with the dilemma of timing and role conflict. Based on the conservation theory, this study explored the relationship between work-family conflict and job satisfaction in 422 female university teachers in China, and proposed a moderating mediation model to examine the mediating role of job burnout and the moderating role of perceived organizational support. The results show that work-family conflict has a significant negative predictive effect on job satisfaction; job burnout fully mediates the relationship between work-family conflict and job satisfaction; perceived organizational support moderates the relationship between work-family conflict and job burnout, and the stronger the perceived organizational support, the weaker the negative predictive effect of work-family conflicts on job satisfaction.