This paper takes work-family conflict as the independent variable, work engagement as the dependent variable, and perceived organizational support and emotional exhaustion as the mediating variables, and studies the influence of work-family conflict on work engagement and the mechanism of perceived organizational support and emotional exhaustion of female university teachers in China, based on the job demand-resource model (JD-R). The results of the questionnaire survey of 518 female university teachers revealed a significant negative relationship between work-family conflict and work engagement, with a direct effect accounting for 31.88%. The indirect effect of work-family conflict on work engagement through perceived organizational support was significant, accounting for 63.19%, while the mediating effect of emotional exhaustion was not notable and the chain mediating influence of perceived organizational support and emotional exhaustion was not significant.