E-learning is a modern and effective approach for training in various areas and at different levels of education. This paper gives an overview of SyRoTek, an e-learning platform for mobile robotics, artificial intelligence, control engineering, and related domains. SyRoTek provides remote access to a set of fully autonomous mobile robots placed in a restricted area with dynamically reconfigurable obstacles, which enables solving a huge variety of problems. A user is able to control the robots in real time by their own developed algorithms as well as being able to analyze gathered data and observe activity of the robots by provided interfaces. The system is currently used for education at the Czech Technical University in Prague, Prague, Czech Republic, and at the University of Buenos Aires, Buenos, Aires, Argentina, and it is freely accessible to other institutions. In addition to the system overview, this paper presents the experience gained from the actual deployment of the system in teaching activities.